HPLC Separation of Four Aromatic Acids on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 20% ACN with 100 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic acid, Catecholamine, Neurotransmitter, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Vanillylmandelic Acid, Mandelic acid, Atrolactic acid, α-Methoxyphenylacetic acid