HPLC Separation of Four Catecholamines on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions

Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 40% ACN with 40 mM AmFm pH 3
Detection: UV 250 nm
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic base, Catecholamine, Drug, Neurotransmitter, Stimulant
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Epinephrine, Norepinephrine, Dopamine, Ephedrine