HPLC Separation of Four Catecholamines on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions
Conditions of Experiment
Column: | Amaze SC |
Separation Modes: | reversed-phase cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5 μm, 100A |
Mobile Phase: | 40% ACN with 40 mM AmFm pH 3 |
Detection: | UV 250 nm |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Aromatic base, Catecholamine, Drug, Neurotransmitter, Stimulant |
Nature of compounds: | Basic, Hydrophilic, Polar |
Compounds: | Epinephrine, Norepinephrine, Dopamine, Ephedrine |