HPLC Separation of Glucose, Sorbitol and Meglumine on Amaze HD HPLC Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 4.6x250 mm, 5 um, 100A
Mobile Phase: 88% ACN with 0.24% TFA
Detection: ELSD
Sample: 0.5-1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aminoglycoside, Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Glucose, Sorbitol, Meglumine