HPLC Separation of Hydrophilic Quaternary Amines (Betaine, Choline and Acethylcholine) on Amaze SC Mixed-Mode Column
Conditions of Experiment
| Column: | Amaze SC |
| Separation Modes: | reversed-phase cation-exchange |
| Column Dimenstions: | 3.0 x 100 mm, 5 μm, 100A |
| Mobile Phase: | 15% ACN with 0.15% TFA |
| Detection: | Corona CAD |
| Sample: | 0.3 m/min |
| Injection: | 2 μl |
| Flow rate: | 0.6 ml/min |
Analytes
| Class of compounds: | Amines, Amino acid, Drug, Neurotransmitter, Supplement, Vitamin |
| Nature of compounds: | Basic, Hydrophilic, Hydrophobic, Zwitterionic |
| Compounds: | Betaine, Choline, Acetylcholine |