HPLC Separation of Isomers of Aminobenzoic Acids on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions
Conditions of Experiment
Column: | Amaze SC |
Separation Modes: | reveresed-phase cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5 um, 100A |
Mobile Phase: | ACN/water/ammonium formate |
Detection: | UV 250 nm |
Sample: | 0.3 mg/ml |
Injection: | 2 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amino acid, Aromatic compound, Isomer, Pyridine |
Nature of compounds: | Hydrophilic, Polar, Zwitterionic |
Compounds: | 4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid |