HPLC Separation of Isomers of Aminobenzoic Acids on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions

Conditions of Experiment
Column: Amaze SC
Separation Modes: reveresed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: ACN/water/ammonium formate
Detection: UV 250 nm
Sample: 0.3 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Amino acid, Aromatic compound, Isomer, Pyridine
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: 4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid