HPLC Separation of Isomers of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC/Mixed-Mode on Amaze HD Column
Conditions of Experiment
Column: | Amaze HA |
Separation Modes: | hydrophilic acidic |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | from 85% ACN with 30 mM Ammonium Acetate pH 4 to 50% ACN with 10 mM Ammonium Acetate pH 4 in 10 min |
Detection: | 280 nm |
Sample: | 0.2 mg/ml |
Injection: | 2 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Aromatic acid, Metabolite, Organic acid, Supplement, Vitamin |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Isoascorbic acid, Ascorbic acid, Ascorbic acid 2-phosphate |