Home HPLC Separation of Isomers of Ascorbic Acid in HILIC and Anion-Exclusion Modes on Amaze HD Column
Conditions of Experiment
| Column: |
Amaze HD |
| Separation Modes: |
HILIC, anion-exchange |
| Column Dimenstions: |
3.0x100 mm, 3 um, 100A |
| Mobile Phase: |
85% ACN with 20 mM Ammonium Acetate pH 4 |
| Detection: |
280 nm |
| Sample: |
0.2 mg/ml |
| Injection: |
2 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Organic acid, Supplement, Vitamin |
| Nature of compounds: |
Acidic, Hydrophilic, Polar |
| Compounds: |
Isoascorbic acid, Ascorbic acid |
