HPLC Separation of Isomers of Ascorbic Acid in HILIC and Anion-Exclusion Modes on Amaze HD Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 85% ACN with 20 mM Ammonium Acetate pH 4
Detection: 280 nm
Sample: 0.2 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Isoascorbic acid, Ascorbic acid