Home HPLC Separation of Isomers of Toluidine on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions
Conditions of Experiment
| Column: |
Amaze SC |
| Separation Modes: |
reveresed-phase cation-exchange |
| Column Dimenstions: |
3.0x100 mm, 5 um, 100A |
| Mobile Phase: |
40% ACN with 40 mM AmFm pH 3 |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
2 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Amines, Aromatic base, Isomer |
| Nature of compounds: |
Basic, Hydrophobic, Polar |
| Compounds: |
m-Toluidine, o-Toluidine, p-Toluidine |
