HPLC Separation of Isomers of Toluidine on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions

Conditions of Experiment
Column: Amaze SC
Separation Modes: reveresed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 um, 100A
Mobile Phase: 40% ACN with 40 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Aromatic base, Isomer
Nature of compounds: Basic, Hydrophobic, Polar
Compounds: m-Toluidine, o-Toluidine, p-Toluidine