HPLC Separation of Leucine and Isoleucine in HILIC, Cation-Exchange Modes on Amaze TCH Column

HPLC-Separation-of-Leucine-and-Isoleucine-in-HILIC,-Cation-Exchange-Modes-on-Amaze-TCH-Column
Amaze TCH PURCHASE LEARN ABOUT Amaze TCH
Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 75% ACN with 15 mM AmFm pH 2.7
Detection: ELSD
Sample: 0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Supplement
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: Leucine, Isoleucine