HPLC Separation of Mono-, Di- and Trisaccharides on Amaze HD HILIC Mixed-Mode Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: ACN/water/AmAc pH 4
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Sugar
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Xylose, Fructose, Glucose, Sucrose, Maltose, Lactose, Melezitose, Raffinose