HPLC Separation of Nucleobases and Nucleosides on Amaze TCH HILIC Mixed-Mode Column Amaze TCH PURCHASE LEARN ABOUT Amaze TCH Conditions of Experiment Column: Amaze TCH Separation Modes: HILIC, cation-exchange Column Dimenstions: 4.6x150 mm, 3 um, 100A Mobile Phase: ACN from 92% to 70% in 15 min, 10 mM AmAc pH 5 Detection: UV 255 nm Sample: 0.2-0.5 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic base, Nucleobase, Nucleoside Nature of compounds: Basic, Hydrophilic, Neutral, Polar Compounds: Thymine, Uracil, Thymidine, Uridine, Adenine, Adenosine, Cytosine, Inosine, Guanine, Cytidine, Guanosine
