HPLC Separation of Nucleobases and Nucleosides on Amaze TCH HILIC Mixed-Mode Column

Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC, cation-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: ACN from 92% to 70% in 15 min, 10 mM AmAc pH 5
Detection: UV 255 nm
Sample: 0.2-0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic base, Nucleobase, Nucleoside
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Thymine, Uracil, Thymidine, Uridine, Adenine, Adenosine, Cytosine, Inosine, Guanine, Cytidine, Guanosine