HPLC Separation of One and Two Valency Metal Ions on Amaze SC Mixed-Mode Column

Conditions of Experiment
Column: Amaze SC
Separation Modes: HILIC cation-exchange
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: A: 80% ACN with 0.15% TFA B: 50% ACN with 0.35% TFA from 100% A to 100% B in 15 min
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Inorganic anion, Inorganic cation
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Lithium ion, Sodium ion, Potassium ion, Magnesium ion, Barium ion, Calcium ion