HPLC Separation of Sugar Phosphates on Amaze HD Mixed-Mode Column

Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, anion-exclusion
Column Dimenstions: 4.6x150 mm, 3 μm, 100A
Mobile Phase: ACN/water/AmAc pH 5
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 1 ml/min
Class of compounds: Inorganic cation, Organic acid, Sugar
Nature of compounds: Acidic, Basic, Hydrophilic, Neutral, Polar
Compounds: Glucose, Fructose-6-phosphate, Glucose-6-phosphate, Fructose-1,6-diphosphate, Sodium ion