Quantitative UV HPLC Analysis of Biosynthetic Ferruginol on Amaze C18 PFP C0lumn

Application description

Biosynthetic ferruginol is a naturally occurring diterpene compound with significant pharmacological and biological activities. Its quantitative analysis is essential for various research and pharmaceutical applications. High-Performance Liquid Chromatography (HPLC) coupled with UV detection offers a reliable method for the accurate determination of biosynthetic ferruginol. In this study, we present a method for the quantitative UV HPLC analysis of biosynthetic ferruginol utilizing an Amaze C18 PFP column. Biosynthetic ferruginol is a diterpene compound derived from plant sources, notably conifer trees. It exhibits diverse biological activities, including anti-inflammatory, antimicrobial, and anticancer properties. Biosynthetic ferruginol is characterized by its hydrophobic nature and structural complexity, making its accurate quantification challenging yet crucial for various research endeavors. The Amaze C18 PFP column incorporates a C18 stationary phase modified with pentafluorophenyl (PFP) groups. This hybrid stationary phase offers unique selectivity and enhanced retention for analytes with diverse polarities and functionalities. The C18 component provides hydrophobic interactions, while the PFP moiety introduces additional π-π interactions, dipole-dipole interactions, and hydrogen bonding capabilities. These combined interactions facilitate efficient separation and resolution of complex mixtures, including biosynthetic ferruginol and related compounds. The application of the Amaze C18 PFP column in UV HPLC analysis provides an effective method for the quantitative determination of biosynthetic ferruginol. This approach offers high sensitivity, selectivity, and reproducibility, making it suitable for various research, pharmaceutical, and biomedical applications. Moreover, the versatility of the Amaze C18 PFP column ensures robust separation and analysis of a wide range of terpenoid compounds, further enhancing its utility in natural product research and drug discovery endeavors.

Conditions of Experiment
Column: Amaze C18 PFP
Separation Modes: reversed-phase
Column Dimenstions: 4.6x150 mm, 3 um, 100 A
Mobile Phase: 65% MeOH with 0.1% perchloric acid
Detection: 255 nm
Sample: 0.1-0.1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Preservative, Terpene
Nature of compounds: Hydrophobic, Neutral
Compounds: Ferruginol