Home Quantitative UV HPLC Analysis of Trifluoracetic Acid in Peptide/Protein on Amaze HA Column
Conditions of Experiment
| Column: |
Amaze HA |
| Separation Modes: |
HILIC, anion-exchage |
| Column Dimenstions: |
3.0x100 mm, 3 um, 100A |
| Mobile Phase: |
60% ACN with 0.1% sulfuric acid |
| Detection: |
215 nm |
| Sample: |
0.1-2 mg/ml |
| Injection: |
2 uL |
| Flow rate: |
0.6 ,ml/min |
Analytes
| Class of compounds: |
Organic acid |
| Nature of compounds: |
Acidic, Hydrophilic, Polar |
| Compounds: |
Trifluoroacetic acid |
