Secondary Interactions in Reversed-Phase Cation-Exchange Mixed-Mode and Reversed-Phase Chromatography

Conditions of Experiment
Column: Amaze SC
Separation Modes: RP, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: ACN/water/phosphoroic acid
Detection: 255 nm
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Organic acid
Nature of compounds: Acidic, Hydrophilic
Compounds: 4-Carboxyphenylboronic acid, Benzoic acid