Secondary Interactions in Reversed-Phase Cation-Exchange Mixed-Mode and Reversed-Phase Chromatography
Conditions of Experiment
Column: | Amaze SC |
Separation Modes: | RP, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 μm, 100A |
Mobile Phase: | ACN/water/phosphoroic acid |
Detection: | 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Aromatic acid, Organic acid |
Nature of compounds: | Acidic, Hydrophilic |
Compounds: | 4-Carboxyphenylboronic acid, Benzoic acid |