Secondary Interactions in Reversed-Phase Cation-Exclusion Mixed-Mode and Reversed-Phase Chromatography

Conditions of Experiment
Column: Heritage MA
Separation Modes: RP, cation-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: ACN/water/TFA
Detection: 255 nm
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic base, Aromatic compound, Surfactant
Nature of compounds: Basic, Hydrophobic
Compounds: Cetylpyridinium, Biphenyl, Phenanthrene