Application description
Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms - reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase |
| Column Dimenstions: |
3.2 x 50 mm, 2.7 um, 90A |
| Mobile Phase: |
20% ACN with 0.1% TFA |
| Detection: |
UV 250 nm, ELSD |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine |
| Nature of compounds: |
Acidic, Hydrophilic, Neutral |
| Compounds: |
Acetaminophen, Caffeine, Benzoic acid |
