Selectivity Difference for Polar Compounds in Reversed-Phase and Mixed-Mode Separation on Coresep 100 HPLC Column

Application description

Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms - reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase
Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A
Mobile Phase: 20% ACN with 0.1% TFA
Detection: UV 250 nm, ELSD
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine
Nature of compounds: Acidic, Hydrophilic, Neutral
Compounds: Acetaminophen, Caffeine, Benzoic acid