Selectivity Difference for Polar Compounds in Reversed-Phase and Mixed-Mode Separation on Coresep 100 HPLC Column
Application description
Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms - reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 3.2 x 50 mm, 2.7 um, 90A |
Mobile Phase: | 20% ACN with 0.1% TFA |
Detection: | UV 250 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Neutral |
Compounds: | Acetaminophen, Caffeine, Benzoic acid |