Application description
Catecholamines (neurotransmitters) are derivatives of the amino acid tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine. Fast and efficient method with base-line separation was achieved on Coresep 100 column. All compounds are separated by combination of reversed-phase and cation-exchange mechanisms. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase, by adjusting mobile phase composition and by changing pH. The method is fully compatible with mass spectroscopy and can be used for fast analysis of neurotransmitters in biofluids. regular. Method can be used a replacement for analysis of neurotransmitters by UPLC with ion-pairing reagents.
Conditions of Experiment
| Column: |
Coresep 100 |
| Separation Modes: |
reversed-phase and cation-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
ACN gradient from 5% to 10% in 5 min, buffer gradient - Ammonium formate pH 2.9 from 5 to 25 mM in 5 min |
| Detection: |
UV 270 nm, ELSD |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Supplement |
| Nature of compounds: |
Basic, Hydrophilic, Polar, Zwitterionic |
| Compounds: |
Dopamine, Tyrosine, Phenylalanine, Norepinephrine, Epinephrine, DOPA |
