Application description
Core-shell columns are able to handle higher flow-rates, while maintaining high efficiency and low back-pressure. Coresep SB was used to achieve separation of nine nucleotides. The strong embedded basic ion-pairing groups make it possible to separate compounds that don’t retain on reverse-phase columns.
Conditions of Experiment
| Column: |
Coresep SB |
| Separation Modes: |
reversed-phase, anion-exchange |
| Column Dimenstions: |
4.6 x 150 mm, 5 um, 100A |
| Mobile Phase: |
5% ACN, AmAc buffer (pH 4.5) gradient from 20 mmol to 300 mmol in 45 minutes |
| Detection: |
UV 270 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
1.5 ml/min |
Analytes
| Class of compounds: |
Amino acid, Aromatic acid, Aromatic base, Nucleoside |
| Nature of compounds: |
Acidic, Basic, Hydrophilic, Polar |
| Compounds: |
Cytidine, Guanosine monophosphate, Guanosine diphosphate, Guanosine triphosphate, Adenosine |
