Simultaneous HPLC Analysis of Drug Fosfomycin and Counterions on Amaze TH Mixed-Mode Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm 5 um, 100A
Mobile Phase: 40% ACN with 20 mM AmAc pH 4
Detection: ELSD
Sample: 1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Antibiotic, Drug, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Fosfomycin, Sodium ion, Tromethamine