Simultaneous HPLC Analysis of Sodium Ion and Sulfate Ion on Amaze TH Mixed-Mode Column

Application description

Ability to retain and separate compounds of opposite charge on one column with one method is important for analysis of salts, drugs, waste waters and other samples. We have developed a method for analysis of sodium and sulfate ions on Amaze TH column. Amaze TH column is HILIC mixed-mode column with both positive and negative sites on the surface. Addition of ionizable groups on the surface allows to increase a polarity of the stationary phase and also make these sites available for ion-exchange interactions. Both ions are well retained with good peak shape. Column and method can be used for analysis of other cations and anions in one run. Method is LC/MS compatible and provides unique selectivity and separation ability for polar, acidic and basic organic and inorganic compounds

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 4.6 x 50 mm, 5 um, 100A
Mobile Phase: 80% ACN with 20 mM AmFm pH 3
Detection: ELSD
Sample: 0.5 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Sodium ion, Sulfate ion