Application description
Ability to retain and separate compounds of opposite charge on one column with one method is important for analysis of salts, drugs, waste waters and other samples. We have developed a method for analysis of sodium and sulfate ions on Amaze TH column. Amaze TH column is HILIC mixed-mode column with both positive and negative sites on the surface. Addition of ionizable groups on the surface allows to increase a polarity of the stationary phase and also make these sites available for ion-exchange interactions. Both ions are well retained with good peak shape. Column and method can be used for analysis of other cations and anions in one run. Method is LC/MS compatible and provides unique selectivity and separation ability for polar, acidic and basic organic and inorganic compounds
Conditions of Experiment
| Column: |
Amaze TH |
| Separation Modes: |
HILIC, anion-exchange |
| Column Dimenstions: |
4.6 x 50 mm, 5 um, 100A |
| Mobile Phase: |
80% ACN with 20 mM AmFm pH 3 |
| Detection: |
ELSD |
| Sample: |
0.5 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Inorganic acid, Inorganic anion, Inorganic cation |
| Nature of compounds: |
Acidic, Basic, Hydrophilic, Polar |
| Compounds: |
Sodium ion, Sulfate ion |
