Simultaneous UV HPLC Analysis of Azide Ion and Toluene on Heritage MA Mixed-Mode Column

Application description

Sodium azide is an inorganic ionic compound which is used in organic synthesis and as a useful probe reagent and preservative. Toluene is an organic solvent used in chemical synthesis. Toluene is neutral and hydrophobic in nature. Due to the completely different nature of these two compounds, developing a single robust method presents serious challenges. These challenges were overcome by using the reversed-phase anion-exchange mixed-mode column Heritage MA. The Azide anion is retained by an anion-exchange mechanism and toluene is retained by a reversed-phase mechanism. The retention of toluene is controlled by the amount of acetonitrile and retention of azide anion is controlled by the amount of buffer and buffer pH. The elution of both compounds is monitored by UV. This method is robust and reproducible. It is compatible with mass spectrometry and other detection techniques. The Heritage MA column can be used for the analysis of hydrophobic neutral, hydrophobic acidic, hydrophobic basic and hydrophilic acidic compounds. Most of the hydrophilic cations will elue* before void

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: ACN./water/AmAc
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Class of compounds: Aromatic compound, Inorganic anion, Organic acid, Organic solvent
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Toluene, Azide Ion