UV HPLC Analysis of Cysteine, Carbocystein and Cystine on Coresep 100 Mixed-Mode Column

Conditions of Experiment
Column: Coresep 100
Separation Modes: reveresed-phase cation-exchange
Column Dimenstions: 4.6x150 mm, 5 um, 100A
Mobile Phase: ACN/water/phosphoric acid gradinet
Detection: UV 230 nm
Sample: 0.5 mg/ml
Flow rate: 1 ml/min
Class of compounds: Amino acid, Supplement
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Cysteine, Carbocysteine, Cystine