Application description
This HPLC method presents the analysis of diabetic drugs Empagliflozin and Metformin utilizing an Amaze SC Mixed-Mode Column. The separation is conducted in both reversed-phase and cation-exchange modes, ensuring baseline separation of both compounds. Order of elution for both compounds can be changed by exploring differences in hydrophobic and ionic properties of Empagliflozin and Metformin.
Empagliflozin is a sodium-glucose cotransporter-2 (SGLT2) inhibitor used in the treatment of type 2 diabetes mellitus. It is a hydrophilic compound with moderate water solubility. Empagliflozin contains an aromatic ring structure with multiple functional groups, including hydroxyl and glucose moiety, which contribute to its hydrophilic/hydrophobic nature.
Metformin is a biguanide antidiabetic medication commonly prescribed to control blood sugar levels in individuals with type 2 diabetes. It is highly water-soluble and possesses basic properties due to the presence of amino groups. Metformin contains a hydrophilic amino group and exhibits a moderate degree of polarity and basicity.
The Amaze SC Mixed-Mode Column is selected for its dual functionality in reversed-phase and cation-exchange modes. In reversed-phase mode, the hydrophobic interactions between the analytes and the stationary phase enable separation based on differences in hydrophobicity. In cation-exchange mode, the column facilitates selective retention and separation of positively charged species, such as metformin, based on ionic interactions.
By employing this HPLC method, precise quantification and characterization of Empagliflozin and Metformin can be achieved, providing valuable insights into their pharmaceutical formulations and therapeutic efficacy in managing diabetes.
Conditions of Experiment
| Column: |
Amaze SC |
| Separation Modes: |
reversed-phase, cation-exchange |
| Column Dimenstions: |
4.6x150 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/water/sulfuric acid |
| Detection: |
235 nm |
| Sample: |
0.3-0.3 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Drug |
| Nature of compounds: |
Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
| Compounds: |
Empagliflozin, Metformin |
