2,4,5-T
2,4,5-T
2,4,5-T
Select Applications
HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column
HPLC Analysis of Herbicide 2,4,5-T on Coresep 100 Mixed-Mode Column
Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
HPLC Analysis of Herbicide 2,4,5-T on Heritage MA Mixed-Mode Column
HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column
Conditions of Experiment
Column: | Coresep SB |
Separation Modes: | reveresed-phase and anion-exchange |
Column Dimenstions: | 4.6x150 mm, 2.7 μm, 90A |
Mobile Phase: | "ACN from 40% to 60% in 15 min, 5 min hold, 0.1% sulfuric acid |
Detection: | 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Aromatic acid, Aromatic compound, Fungicide, Herbicide, Organic acid, Pesticide |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Dalapon, 2,4-DB, Dinoseb, MCPA, Mecoprop, Dichlorprop, 2,4-D, Picloram, Dicamba, Silvex, 2,4,5-T, 3,5-DCBA, Pentachlorophenol (PCP) |
HPLC Analysis of Herbicide 2,4,5-T on Coresep 100 Mixed-Mode Column
Application description
2,4,5- Trichlorophenoxyacetic acid or 2,4,5-T is chlorophenoxy acetic herbicide used to defoliate broad-leafed plants. It is hydrophobic and acidic in nature and was analyzed on Coresep 100 mixed-mode column in reversed-phase and anion-exchange modes. Retention time can be adjusted by the amount of ACN, buffer concentration and buffer pH. This method demonstrates good retention control and perfect peak shape for this acidic and hydrophobic analyte. The method is compatible with major detection techniques (UV, ELSD, CAD, RI, and MS).
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase and anion-exclusion |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Aromatic acid, Herbicide, Pesticide |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | 2,4,5-T |
Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold |
Detection: | UV 275 nm |
Sample: | 0.1-0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide, Pyridine |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Clopyralid (herbicide), Sulfadiazine, Sulfamethazine, Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, MCPA, Thiacloprid (insecticide), 2,4,5-T, Diphenamid, Captan (fungicide), 2,4-DB, Triadimefon, Azinphos Ethyl, Diazinon, Chlorpyrifos Methyl, Phoxim, Teramisol (antibiotic), Benfluralin (herbicide), Dicofol, Hexachlorobenzene |
Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold |
Detection: | UV 275 nm |
Sample: | 0.1-0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Clopyralid (herbicide), Sulfadiazine, Aniline (herbicide precursor), Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, Diphenamid, 2,4,5-T, Captan (fungicide), 2,4-DB, Folpet, Diazinon, Azinphos Ethyl, Chlorpyrifos Methyl, Phoxim, Benfluralin (herbicide), Dicofol, Hexachlorobenzene |
HPLC Analysis of Herbicide 2,4,5-T on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: | Heritage MA |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 4.6 x 50 mm |
Mobile Phase: | 60% ACN with 80 mM AmFm pH 3 |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 mL/min |
Analytes
Class of compounds: | Aromatic acid, Herbicide, Pesticide |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | 2,4,5-T |