2′-Deoxyadenosine

2-Deoxyadenosine C10H13N5O3

2′-Deoxyadenosine

Applications

HPLC Method for Analysis of Nucleosides and Nucleoside Derivatives on Amaze HD Column

HPLC Method for Analysis of Nucleosides and Nucleoside Derivatives on Amaze HD Column chromatogram
Conditions of Experiment
Column:
Amaze HD
Separation Modes:
hydrogen-bonding and cation-exchange
Column Dimenstions: 3.2 x 100 mm
Mobile Phase: MeCN/MeOH from 90/10 to 50/50 with 0.1% HCOOH and 0.01% AmFm in 8 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.8 mL/min
Analytes
1. Thymidine
2. Uridine
3. 2′-Deoxyadenosine
4. Adenosine
5. Guanosine

Class of compounds: Aromatic base, Nucleoside
Nature of compounds: Basic, Hydrophilic, Neutral, Polar

Application description

Thymidine, uridine, deoxyadenosine, adenosine, deoxyguanosine and guanosine were separated on an Amaze HD column with LC/MS compatible method. The approach can be used to analyze nucleotides, nucleosides and their derivatives in various sample matrices. This HPLC method is robust and reproducible, and can be used for other compounds that can form hydrogen bonds with stationary phase and interact by electrostatic interaction.

HPLC Method for Analysis of Inosine and Deoxyinosine on Amaze HD Column

HPLC Method for Analysis of Inosine and Deoxyinosine on Amaze HD Column chromatogram
Conditions of Experiment
Column:
Amaze HD
Separation Modes:
HILIC
Column Dimenstions: 3.2 x 50 mm
Mobile Phase: MeCN/MeOH (97/3) with 0.1% HCOOH and 0.01% AmFm
Detection: 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.0 mL/min
Analytes
1. 2′-Deoxyadenosine
2. Inosine

Class of compounds: Nucleoside
Nature of compounds: Hydrophilic, Neutral, Polar

Application description

Inosine is a nucleoside containing hypoxanthine and ribose molecules. Deoxyinosine is a nucleoside containing hypoxanthine and deoxyribose molecules. Both molecules are polar and slightly basic. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, and CAD.

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