Application description
Thymidine, uridine, deoxyadenosine, adenosine, deoxyguanosine and guanosine were separated on an Amaze HD column with LC/MS compatible method. The approach can be used to analyze nucleotides, nucleosides and their derivatives in various sample matrices. This HPLC method is robust and reproducible, and can be used for other compounds that can form hydrogen bonds with stationary phase and interact by electrostatic interaction.
Conditions of Experiment
| Column: |
Amaze HD |
| Separation Modes: |
hydrogen-bonding and cation-exchange |
| Column Dimenstions: |
3.2 x 100 mm |
| Mobile Phase: |
MeCN/MeOH from 90/10 to 50/50 with 0.1% HCOOH and 0.01% AmFm in 8 min |
| Detection: |
UV 275 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
1 uL |
| Flow rate: |
0.8 mL/min |
Analytes
| Class of compounds: |
Aromatic base, Nucleoside |
| Nature of compounds: |
Basic, Hydrophilic, Neutral, Polar |
| Compounds: |
Thymidine, Uridine, 2′-Deoxyadenosine, Adenosine, Guanosine |
