2,4-D

2,4-D (2,4-Dichlorphenoxyacetic acid) C8H6Cl2O3

Select Applications HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column HPLC Analysis of Herbicide 2,4-D on Coresep 100 Mixed-Mode Column Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column HPLC Analysis of Herbicide 2,4-D on Heritage MA Mixed-Mode Column

HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column

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Conditions of Experiment

Column:	Coresep SB
Separation Modes:	reveresed-phase and anion-exchange
Column Dimenstions:	4.6x150 mm, 2.7 μm, 90A
Mobile Phase:	"ACN from 40% to 60% in 15 min, 5 min hold, 0.1% sulfuric acid
Detection:	275 nm
Sample:	0.3 mg/ml
Injection:	2 μL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aromatic acid, Aromatic compound, Fungicide, Herbicide, Organic acid, Pesticide
Nature of compounds:	Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Dalapon, 2,4-DB, Dinoseb, MCPA, Mecoprop, Dichlorprop, 2,4-D, Picloram, Dicamba, Silvex, 2,4,5-T, 3,5-DCBA, Pentachlorophenol (PCP)

HPLC Analysis of Herbicide 2,4-D on Coresep 100 Mixed-Mode Column

Application description

2,4-D or 2,4-dichlorophenoxyacetic acid is phenoxyacetic herbicide used in the treatment of broadleaf weeds. 2,4-D is one of the most widely used herbicides and defoliant. 2,4-D is manufactured from chloroacetic acid and 2,4-dichlorophenol. 2,4-D is often used in combination with dicamba, mecoprop, and MCPA. 2,4-D was analyzed on core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by the amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions. Core-shell mixed-mode chromatography offers a synergy of unique selectivity of mixed-mode stationary phases and high efficiency of core-shell particles.

HPLC Analysis of Herbicide 2,4-D on Coresep 100 Mixed-Mode Column chromatogram

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase and anion-exclusion
Column Dimenstions:	3.0 x 100 mm
Mobile Phase:	ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection:	UV 275 nm
Sample:	0.3 mg/ml
Injection:	1 uL
Flow rate:	0.7 mL/min

Analytes

Class of compounds:	Aromatic acid, Herbicide, Pesticide
Nature of compounds:	Acidic, Hydrophilic, Polar
Compounds:	2,4-D

Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions:	3.0 x 100 mm
Mobile Phase:	ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection:	UV 275 nm
Sample:	0.1-0.3 mg/ml
Injection:	1 uL
Flow rate:	0.7 mL/min

Analytes

Class of compounds:	Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide, Pyridine
Nature of compounds:	Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds:	Clopyralid (herbicide), Sulfadiazine, Sulfamethazine, Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, MCPA, Thiacloprid (insecticide), 2,4,5-T, Diphenamid, Captan (fungicide), 2,4-DB, Triadimefon, Azinphos Ethyl, Diazinon, Chlorpyrifos Methyl, Phoxim, Teramisol (antibiotic), Benfluralin (herbicide), Dicofol, Hexachlorobenzene

Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions:	3.0 x 100 mm
Mobile Phase:	ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection:	UV 275 nm
Sample:	0.1-0.3 mg/ml
Injection:	1 uL
Flow rate:	0.7 mL/min

Analytes

Class of compounds:	Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide
Nature of compounds:	Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds:	Clopyralid (herbicide), Sulfadiazine, Aniline (herbicide precursor), Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, Diphenamid, 2,4,5-T, Captan (fungicide), 2,4-DB, Folpet, Diazinon, Azinphos Ethyl, Chlorpyrifos Methyl, Phoxim, Benfluralin (herbicide), Dicofol, Hexachlorobenzene

HPLC Analysis of Herbicide 2,4-D on Heritage MA Mixed-Mode Column

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Conditions of Experiment

Column:	Heritage MA
Separation Modes:	reversed-phase and anion-exchange
Column Dimenstions:	4.6 x 50 mm
Mobile Phase:	10% ACN with 10 mM AmFm pH 3
Detection:	UV 275 nm
Sample:	0.3 mg/ml
Injection:	1 uL
Flow rate:	1 mL/min

Analytes

Class of compounds:	Aromatic acid, Herbicide, Pesticide
Nature of compounds:	Acidic, Hydrophilic, Polar
Compounds:	2,4-D