2,4-DB
2,4-DB
2,4-DB
Select Applications
HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column
HPLC Analysis of Herbicide 2,4-DB on Coresep 100 Mixed-Mode Column
Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
HPLC Analysis of Herbicide 2,4-DB on Heritage MA Mixed-Mode Column
HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column
Conditions of Experiment
Column: | Coresep SB |
Separation Modes: | reveresed-phase and anion-exchange |
Column Dimenstions: | 4.6x150 mm, 2.7 μm, 90A |
Mobile Phase: | "ACN from 40% to 60% in 15 min, 5 min hold, 0.1% sulfuric acid |
Detection: | 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Aromatic acid, Aromatic compound, Fungicide, Herbicide, Organic acid, Pesticide |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Dalapon, 2,4-DB, Dinoseb, MCPA, Mecoprop, Dichlorprop, 2,4-D, Picloram, Dicamba, Silvex, 2,4,5-T, 3,5-DCBA, Pentachlorophenol (PCP) |
HPLC Analysis of Herbicide 2,4-DB on Coresep 100 Mixed-Mode Column
Application description
2,4-DB or 4-(2,4-dichlorophenoxy)butyric acid is a selective systemic phenylbutyric herbicide used to control annual and perennial broadleaf weeds. 2,4-DB is acidic and hydrophobic in nature and can be analyzed on Coresep 100 core-shell mixed-mode column in reversed-phase anion-exclusion modes. Retention time is controlled by an amount of ACN, buffer concentration and buffer pH. The method is suitable for analysis of various herbicides with LC/MS compatible conditions.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase and anion-exclusion |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Aromatic acid, Herbicide, Pesticide |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | 2,4-DB |
Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold |
Detection: | UV 275 nm |
Sample: | 0.1-0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide, Pyridine |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Clopyralid (herbicide), Sulfadiazine, Sulfamethazine, Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, MCPA, Thiacloprid (insecticide), 2,4,5-T, Diphenamid, Captan (fungicide), 2,4-DB, Triadimefon, Azinphos Ethyl, Diazinon, Chlorpyrifos Methyl, Phoxim, Teramisol (antibiotic), Benfluralin (herbicide), Dicofol, Hexachlorobenzene |
Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase, cation-exchange, anion-exclusion |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold |
Detection: | UV 275 nm |
Sample: | 0.1-0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Clopyralid (herbicide), Sulfadiazine, Aniline (herbicide precursor), Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, Diphenamid, 2,4,5-T, Captan (fungicide), 2,4-DB, Folpet, Diazinon, Azinphos Ethyl, Chlorpyrifos Methyl, Phoxim, Benfluralin (herbicide), Dicofol, Hexachlorobenzene |
HPLC Analysis of Herbicide 2,4-DB on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: | Heritage MA |
Separation Modes: | reversed-phase and anion-exclusion |
Column Dimenstions: | 4.6 x 50 mm |
Mobile Phase: | 45% ACN with 80 mM AmFm pH 3 |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 mL/min |
Analytes
Class of compounds: | Aromatic acid, Herbicide, Pesticide |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | 2,4-DB |