2,4,5-T

HPLC Analysis of Herbicide 2,4,5-T on Coresep 100 Mixed-Mode Column
Application description

2,4,5- Trichlorophenoxyacetic acid or 2,4,5-T is chlorophenoxy acetic herbicide used to defoliate broad-leafed plants. It is hydrophobic and acidic in nature and was analyzed on Coresep 100 mixed-mode column in reversed-phase and anion-exchange modes. Retention time can be adjusted by the amount of ACN, buffer concentration and buffer pH. This method demonstrates good retention control and perfect peak shape for this acidic and hydrophobic analyte. The method is compatible with major detection techniques (UV, ELSD, CAD, RI, and MS).

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
Class of compounds: Aromatic acid, Herbicide, Pesticide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: 2,4,5-T
Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
Class of compounds: Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide, Pyridine
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds: Clopyralid (herbicide), Sulfadiazine, Sulfamethazine, Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, MCPA, Thiacloprid (insecticide), 2,4,5-T, Diphenamid, Captan (fungicide), 2,4-DB, Triadimefon, Azinphos Ethyl, Diazinon, Chlorpyrifos Methyl, Phoxim, Teramisol (antibiotic), Benfluralin (herbicide), Dicofol, Hexachlorobenzene
Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column
Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection: UV 275 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.7 mL/min
Analytes
Class of compounds: Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds: Clopyralid (herbicide), Sulfadiazine, Aniline (herbicide precursor), Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, Diphenamid, 2,4,5-T, Captan (fungicide), 2,4-DB, Folpet, Diazinon, Azinphos Ethyl, Chlorpyrifos Methyl, Phoxim, Benfluralin (herbicide), Dicofol, Hexachlorobenzene
HPLC Analysis of Herbicide 2,4,5-T on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6 x 50 mm
Mobile Phase: 60% ACN with 80 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Aromatic acid, Herbicide, Pesticide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: 2,4,5-T