2,4,5-T

2,4,5-T (2,4,5-Trichlorophenoxyacetic acid) Cl3C6H2OCH2CO2H

Select Applications HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column HPLC Analysis of Herbicide 2,4,5-T on Coresep 100 Mixed-Mode Column Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column HPLC Analysis of Herbicide 2,4,5-T on Heritage MA Mixed-Mode Column

HPLC Analysis of Acidic Herbicides in Reverse-Phase Anion-Exchange Modes on Coresep SB Column

Coresep SB PURCHASE LEARN ABOUT Coresep SB

Conditions of Experiment

Column:	Coresep SB
Separation Modes:	reveresed-phase and anion-exchange
Column Dimenstions:	4.6x150 mm, 2.7 μm, 90A
Mobile Phase:	"ACN from 40% to 60% in 15 min, 5 min hold, 0.1% sulfuric acid
Detection:	275 nm
Sample:	0.3 mg/ml
Injection:	2 μL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aromatic acid, Aromatic compound, Fungicide, Herbicide, Organic acid, Pesticide
Nature of compounds:	Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds:	Dalapon, 2,4-DB, Dinoseb, MCPA, Mecoprop, Dichlorprop, 2,4-D, Picloram, Dicamba, Silvex, 2,4,5-T, 3,5-DCBA, Pentachlorophenol (PCP)

HPLC Analysis of Herbicide 2,4,5-T on Coresep 100 Mixed-Mode Column

Application description

2,4,5- Trichlorophenoxyacetic acid or 2,4,5-T is chlorophenoxy acetic herbicide used to defoliate broad-leafed plants. It is hydrophobic and acidic in nature and was analyzed on Coresep 100 mixed-mode column in reversed-phase and anion-exchange modes. Retention time can be adjusted by the amount of ACN, buffer concentration and buffer pH. This method demonstrates good retention control and perfect peak shape for this acidic and hydrophobic analyte. The method is compatible with major detection techniques (UV, ELSD, CAD, RI, and MS).

HPLC Analysis of Herbicide 2,4,5-T on Coresep 100 Mixed-Mode Column chromatogram

Coresep 100 PURCHASE LEARN ABOUT Coresep 100

Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase and anion-exclusion
Column Dimenstions:	3.0 x 100 mm
Mobile Phase:	ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min
Detection:	UV 275 nm
Sample:	0.3 mg/ml
Injection:	1 uL
Flow rate:	0.7 mL/min

Analytes

Class of compounds:	Aromatic acid, Herbicide, Pesticide
Nature of compounds:	Acidic, Hydrophilic, Polar
Compounds:	2,4,5-T

Baseline HPLC Separation of 22 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column

Coresep 100 PURCHASE LEARN ABOUT Coresep 100

Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions:	3.0 x 100 mm
Mobile Phase:	ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection:	UV 275 nm
Sample:	0.1-0.3 mg/ml
Injection:	1 uL
Flow rate:	0.7 mL/min

Analytes

Class of compounds:	Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide, Pyridine
Nature of compounds:	Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds:	Clopyralid (herbicide), Sulfadiazine, Sulfamethazine, Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, MCPA, Thiacloprid (insecticide), 2,4,5-T, Diphenamid, Captan (fungicide), 2,4-DB, Triadimefon, Azinphos Ethyl, Diazinon, Chlorpyrifos Methyl, Phoxim, Teramisol (antibiotic), Benfluralin (herbicide), Dicofol, Hexachlorobenzene

Baseline HPLC Separation of 19 Acidic, Basic and Neutral Pesticides and Antibiotics on Coresep 100 Mixed-Mode Column

Coresep 100 PURCHASE LEARN ABOUT Coresep 100

Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase, cation-exchange, anion-exclusion
Column Dimenstions:	3.0 x 100 mm
Mobile Phase:	ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min, 3 min hold
Detection:	UV 275 nm
Sample:	0.1-0.3 mg/ml
Injection:	1 uL
Flow rate:	0.7 mL/min

Analytes

Class of compounds:	Amino acid, Antibiotic, Aromatic acid, Aromatic base, Aromatic compound, Drug, Fungicide, Herbicide, Insecticide, Pesticide
Nature of compounds:	Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds:	Clopyralid (herbicide), Sulfadiazine, Aniline (herbicide precursor), Cefepime (antibiotic), Metribuzin, Bromacil, 2,4-D, Diphenamid, 2,4,5-T, Captan (fungicide), 2,4-DB, Folpet, Diazinon, Azinphos Ethyl, Chlorpyrifos Methyl, Phoxim, Benfluralin (herbicide), Dicofol, Hexachlorobenzene

HPLC Analysis of Herbicide 2,4,5-T on Heritage MA Mixed-Mode Column

Heritage MA PURCHASE LEARN ABOUT Heritage MA

Conditions of Experiment

Column:	Heritage MA
Separation Modes:	reversed-phase and anion-exchange
Column Dimenstions:	4.6 x 50 mm
Mobile Phase:	60% ACN with 80 mM AmFm pH 3
Detection:	UV 275 nm
Sample:	0.3 mg/ml
Injection:	1 uL
Flow rate:	1 mL/min

Analytes

Class of compounds:	Aromatic acid, Herbicide, Pesticide
Nature of compounds:	Acidic, Hydrophilic, Polar
Compounds:	2,4,5-T