HPLC Separation of Hydrophilic Amines on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 30% ACN with 50 mM AmFm pH 3
Detection: UV 250 nm
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Aromatic base, Pyridine
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Aniline (herbicide precursor), Pyridine, 2-Aminopyridine, 4-Aminophenol, 2,6-Lutidine