HPLC Method for Analysis of Pyridine and Three Isomers of Aminopyridine on Amaze HD Column
Application description

Pyridine and its derivatives are important building blocks in pharmaceutical and chemical industries. Most pyridines are hydrophilic compounds with pKa around 5.2-6. Hydrophilic nature of these compounds require the use of ion-pairing reagents that are not compatible with LC/MS. We have developed a method with good peak shape and resolution. All compounds are eluted within 6 minutes with isocratic HPLC conditions. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, RI and CAD.

Conditions of Experiment
Column: Amaze HD
Separation Modes: hydrogen-bonding and cation-exchange
Column Dimenstions: 3.2 x 150 mm
Mobile Phase: MeCN/MeOH (60/40) with 0.2% HCOOH and 0.25% AmFm
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1.0 mL/min
Class of compounds: Amines, Aromatic base, Aromatic compound, Isomer, Pyridine
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Pyridine, 2-Aminopyridine, 3-Aminopyridine, 4-Aminopyridine