3-Aminosalicylic acid

HPLC Separation of Three Isomers of Aminosalicylic Acid on Coresep 100 Mixed-Mode Column
Application description

Aminosalicylic acids are organic zwitterionic compounds which are hydrophilic in nature. They are very similar in properties and separation of all three isomers is a challenging task. 3-Aminosalicylic, 4-aminosalicylic and 5-aminosalicylic acids were separated on Coresep 100 core-shell mixed-mode column. Mixed-mode chromatography explores a small difference in hydrophobic and ionic properties of molecules to enhance resolution between isomers and other close related compounds. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of isomers of aminosalicylic acids in various sample matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Isomers of other compounds can be retained and separated if they are hydrophobic and basic in nature.Core-shell mixed-mode columns provide а great combination of unique selectivity, speed, and efficiency.

Conditions of Experiment
Column: Coresep 100
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0 x 100 mm
Mobile Phase: 20% ACN with 0.1% H3PO4
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Amino acid, Aromatic compound, Isomer
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: 3-Aminosalicylic acid, 4-Aminosalicylic acid, 5-Aminosalicylic acid