HPLC Separation of Pyridine, Bromopyridine and Bipyridine on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cation-exchange
Column Dimenstions: 3.0x150 mm, 3 um, 100A
Mobile Phase: ACN/Water/AmFm pH 3 gradient
Detection: 254 nm
Sample: 0.2-0.5 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic base, Pyridine
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Pyridine, 3-Bromopyridine, 3,3′-Bipyridine