4-Aminophenol

HPLC Analysis of 6 Aromatic Acids and Alcohols on Amaze Aromatic Stationary Phases
Application description

Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns.

Conditions of Experiment
Column: Amaze SPF
Separation Modes: reversed-phase, pi-pi
Column Dimenstions: 4.6x150 mm, 5 μm, 100A
Mobile Phase: 35% ACN with 20 mM AmAc pH 4.5
Detection: UV 235 nm
Sample: 1 mg/ml
Injection: 3 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Aromatic acid, Aromatic base, Aromatic compound, Organic acid, Organic compound, Preservative
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: 4-Aminophenol, Mandelic acid, 4-Hydroxybenzoic acid, Phenol, Benzoic acid, Methylparaben
HPLC Analysis of 4-Aminophenol in Acetaminophen Tablets on Amaze SC Mixed-Mode Column in Reversed-Phase and Cation-Exchange Modes
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 2% ACN wiith 50 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Aromatic base, Aromatic compound, Drug
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Acetaminophen, 4-Aminophenol
HPLC Separation of Hydrophilic Amines on Amaze SC Mixed-Mode Column with LC/MS Compatible Conditions
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 30% ACN with 50 mM AmFm pH 3
Detection: UV 250 nm
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amines, Aromatic base, Pyridine
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Aniline (herbicide precursor), Pyridine, 2-Aminopyridine, 4-Aminophenol, 2,6-Lutidine