HPLC Method for Analysis of Pyridine and Three Isomers of Aminopyridine on Amaze HD Column
|Conditions of Experiment|
Class of compounds: Amines, Aromatic base, Aromatic compound, Isomer, Pyridine
|Nature of compounds: Basic, Hydrophilic, Polar|
Pyridine and its derivatives are important building blocks in pharmaceutical and chemical industries. Most pyridines are hydrophilic compounds with pKa around 5.2-6. Hydrophilic nature of these compounds require the use of ion-pairing reagents that are not compatible with LC/MS. We have developed a method with good peak shape and resolution. All compounds are eluted within 6 minutes with isocratic HPLC conditions. Mobile phase allows to use mass spectrometry detection technique as well as UV, ELSD, RI and CAD.