HPLC Separation of Three Isomers of Aminosalicylic Acid on Coresep 100 Mixed-Mode Column
|Conditions of Experiment|
1. 3-Aminosalicylic acid
2. 4-Aminosalicylic acid
3. 5-Aminosalicylic acid
Class of compounds: Amino acid, Aromatic compound, Isomer
|Nature of compounds: Hydrophilic, Polar, Zwitterionic|
Aminosalicylic acids are organic zwitterionic compounds which are hydrophilic in nature. They are very similar in properties and separation of all three isomers is a challenging task. 3-Aminosalicylic, 4-aminosalicylic and 5-aminosalicylic acids were separated on Coresep 100 core-shell mixed-mode column. Mixed-mode chromatography explores a small difference in hydrophobic and ionic properties of molecules to enhance resolution between isomers and other close related compounds. Retention time is controlled by the amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of isomers of aminosalicylic acids in various sample matrices. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection). Isomers of other compounds can be retained and separated if they are hydrophobic and basic in nature.Core-shell mixed-mode columns provide а great combination of unique selectivity, speed, and efficiency.