Acetaminophen

Hydrocodone/paracetamol, also known as hydrocodone/acetaminophen or hydrocodone/APAP is the combination of an opioid pain medication, hydrocodone, with paracetamol (acetaminophen). It is used as a prescription drug to relieve moderate to severe pain. It exists in tablet, elixir, and solution forms in various strengths for oral administration. Vicodin is Schedule II controlled substance. Hydrocodone diversion and recreational use has escalated in recent years due to its opioid effects. Norco was analyzed with isocratic conditions to provide fast, reliable and robust method for analysis of both components of the drug combination - hydrocodone and acetaminophen. Compounds are retained and separated on Heritage MA HPLC column in reversed-phase and cation-exclusion mechanisms. Retention time, selectivity, and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Heritage MA column to accommodate different detection techniques (UV, MS, CAD, ELSD detection).

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Conditions of Experiment

Column:	Heritage MA
Separation Modes:	reversed-phase and cation-exchange
Column Dimenstions:	4.6 x 150 mm
Mobile Phase:	5% ACN with 50 mM AmAc pH 4
Detection:	UV 215 nm
Sample:	0.3 mg/ml
Injection:	3 uL
Flow rate:	1 mL/min

Analytes

Class of compounds:	Aromatic base, Aromatic compound, Controlled substance, Drug
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral
Compounds:	Hydrocodone, Acetaminophen

Codeine/paracetamol, also known as codeine/acetaminophen or hydrocodone/APAP is the combination of an opioid pain medication, codeine, with paracetamol (acetaminophen). It is used as a prescription drug to relieve moderate to severe pain. It exists in tablet, elixir, and solution forms in various strengths for oral administration. Vicodin is Schedule II controlled substance. Codeine diversion and recreational use has escalated in recent years due to its opioid effects. Vicodin was analyzed with isocratic conditions to provide fast, reliable and robust method for analysis of both components of the drug combination - codeine and acetaminophen. Compounds are retained and separated on Heritage MA HPLC column in reversed-phase and cation-exclusion mechanisms. Retention time, selectivity and resolution between peaks can be adjusted by modification of amount of ACN, buffer and buffer pH. Various mobile phases can be used with Coresep 100 column to accommodate different detection techniques (UV, MS, CAD, ELSD detection).

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Conditions of Experiment

Column:	Heritage MA
Separation Modes:	reversed-phase and cation-exclusion
Column Dimenstions:	4.6 x 150 mm
Mobile Phase:	5% ACN with 50 mM AmAc pH 4
Detection:	UV 275 nm
Sample:	0.3 mg/ml
Injection:	3 uL
Flow rate:	1 mL/min

Analytes

Class of compounds:	Aromatic base, Aromatic compound, Controlled substance, Drug
Nature of compounds:	Basic, Hydrophilic, Hydrophobic, Neutral
Compounds:	Codeine, Acetaminophen

Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms - reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.

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Conditions of Experiment

Column:	Coresep 100
Separation Modes:	reversed-phase
Column Dimenstions:	3.2 x 50 mm, 2.7 um, 90A
Mobile Phase:	20% ACN with 0.1% TFA
Detection:	UV 250 nm, ELSD
Sample:	0.3 mg/ml
Injection:	1 uL
Flow rate:	1 ml/min

Analytes

Class of compounds:	Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine
Nature of compounds:	Acidic, Hydrophilic, Neutral
Compounds:	Acetaminophen, Caffeine, Benzoic acid