HPLC Separation of Hydrophilic Quaternary Amines (Betaine, Choline and Acethylcholine) on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 3.0 x 100 mm, 5 μm, 100A
Mobile Phase: 15% ACN with 0.15% TFA
Detection: Corona CAD
Sample: 0.3 m/min
Injection: 2 μl
Flow rate: 0.6 ml/min
Class of compounds: Amines, Amino acid, Drug, Neurotransmitter, Supplement, Vitamin
Nature of compounds: Basic, Hydrophilic, Hydrophobic, Zwitterionic
Compounds: Betaine, Choline, Acetylcholine