Application description
The Arginine-Polyamine metabolic cycle encompasses a series of biochemical reactions critical for cellular functions, including cell growth and differentiation. Key metabolites in this cycle include arginine, agmatine, ornithine, citrulline, carbomoylputrescene, and the polyamines putrescine, spermidine, and spermine. All compounds in a pathway are highly polar and ionic analytes with poor retention and poor peak shape on traditional HILIC and reversed-phase columns. We have developed a simple HILIC mixed-mode approach to analyze compounds in the Arginine-Polyamine metabolic pathway. The method uses low concentrations of ammonium formate and formic acid to achieve highly symmetrical and well-separated peaks. Please contact us for details of this method or to inquire about our Method Development Services.
Conditions of Experiment
| Column: |
Amaze MH |
| Separation Modes: |
HILIC, cation-exchange |
| Column Dimenstions: |
3x100 mm, 3 um, 100 A |
| Mobile Phase: |
ACN/water/ammonium formate/formic acid |
| Detection: |
Mass Spectrometry (MS) |
| Sample: |
various-0.6 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.6 ml/min |
Analytes
| Class of compounds: |
Amines, Amino acid, Aromatic acid, Metabolite, Polyamine, Polymer, Supplement |
| Nature of compounds: |
Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
| Compounds: |
N-Carbomoylputrescene, Citrulline, Agmatine, Arginine, Putrescence, Ornithine, Spermidine, Spermine |
