Alanine methyl ester

HPLC Separation of Alanine and Corresponding Esters on Amaze SC HPLC Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase, cations-exchange
Column Dimenstions: 4.6x50 mm, 3 um, 100A
Mobile Phase: ACN/water/phosphoric acid
Detection: 200 nm
Sample: 0.3-0.5 uL
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Amino acid, Aromatic acid
Nature of compounds: Hydrophilic, Hydrophobic, Polar, Zwitterionic
Compounds: Alanine, Alanine methyl ester, Alanine ethyl ester, Alanine isopropyl ester