Alanine

Alanine C3H7NO2

Alanine

Applications

HPLC Analysis of Histidine and Related Impurities

HPLC Analysis of Histidine and Related Impurities chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
Column Dimenstions: 4.6 x 150 mm, 2.7 um, 90A
Mobile Phase: ACN from 2% to 20%, Na2HPO4 pH 6 from 10 mM to 50 mM in 12 min
Detection: UV 210 nm
Sample:
Injection:
Flow rate: 1 mL/min
Analytes
1. Alanine
2. Histidine
3. Lysine
4. Arginine

Class of compounds: Amino acid, Supplement
Nature of compounds: Hydrophilic, Polar, Zwitterionic

Application description

Ultra-Fast HPLC Separation of Underivatized Amino Acids in Reversed-Phase and Cation-Exchange Modes

Ultra-Fast HPLC Separation of Underivatized Amino Acids in Reversed-Phase and Cation-Exchange Modes chromatogram
Conditions of Experiment
Column:
Coresep 100
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 3.2 x 50 mm, 2.7 um, 90A
Mobile Phase: 3% ACN with 0.03% TFA
Detection: UV 210 nm, ELSD
Sample: 0.3 mg/ml
Injection: 10 uL
Flow rate: 1 ml/min
Analytes
1. Glycine
2. Alanine
3. Serine
4. Valine
5. Dopamine

Class of compounds: Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic

Application description

Amino acids are essential components of numerous formulation. Health supplements can contain various amino acids and vitamins and require quantitation of each ingredients. Amino acids are very polar compounds with limited or no retention in reversed-phase chromatography. The most common approaches are reversed-phase chromatography with ion-pairing reagent and hydrophilic interaction chromatography (HILIC). Underivatized amino acids can be retained by combination of reversed-phase and cation exchange mechanism. These  two mechanism were combined in Coresep 100 core-shell mixed-mode column. Method can be used for fast analysis of underivatized amino acids without ion-pairing reagent. Depending on the pH of the mobile phase they can exist in acidic, basic or zwitter-ionic form. At lower pH, amino acids are basic in nature and have the highest hydrophobicity. Four amino acids (SER, GLY, ALA, VAL and DOPA) were retained and separated within 2 minutes on core-shell mixed-mode column. This high efficiency separation does not require special UPLC set up and can benefit from core-shell and mixed-mode approaches.

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