Arginine

HPLC Analysis of Dopamine and 5 Amino Acids in HILIC and Cation-Exchange Modes on Amaze HD Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange and anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: 72% ACN with 56 mM AmFm pH 3
Detection: Corona CAD
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine
HPLC Analysis of Dopamine and 5 Amino Acids in Reversed-Phase and Cation-Exchange Modes on Amaze SC Column
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase and cation-exchange
Column Dimenstions: 3.0x100 mm, 5μm, 100A
Mobile Phase: 10% ACN with 20 mM AmFm pH 3
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement
Nature of compounds: Acidic, Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine
HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes
Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 4.6x250 mm, 5 μm, 100A
Mobile Phase: A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold
Detection: Corona CAD
Sample: 0.2 mg/ml
Injection: 5 μL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amino acid, Neurotransmitter, Supplement
Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic
Compounds: Aspartic acid, Serine, Asparagine, Cysteine, Glutamic acid, Threonine, Glutamine, Glycine, Alanine, Proline, Valine, Methionine, Tyrosine, Isoleucine, Leucine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine
HPLC Analysis of Sucrose, Histidine and Arginine on Amaze HD Mixed-Mode Column
Conditions of Experiment
Column: Amaze HD
Separation Modes: HILIC, cation-exchange, anion-exclusion
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 50 mM AmAc pH 5
Detection: ELSD, 40°C
Sample: 2 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Amino acid, Inorganic anion, Sugar, Supplement
Nature of compounds: Acidic, Hydrophilic, Neutral, Polar, Zwitterionic
Compounds: Chloride Ion, Sucrose, Histidine, Arginine