Aspirin
HPLC Analysis of 6 Aromatic Acidic, Zwitterionic and Neutral Compounds on Amaze Aromatic Stationary Phases
Application description
Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns.
Conditions of Experiment
Column: | Amaze AR |
Separation Modes: | reversed-phase, pi-pi |
Column Dimenstions: | 4.6x150 mm, 5 μm, 100A |
Mobile Phase: | 28% ACN with 20 mM AmAc pH 4.5 |
Detection: | UV 255 nm |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Amino acid, Aromatic acid, Aromatic compound, Drug, Isomer, Organic acid, Preservative, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Caffeine, 4-Aminobenzoic acid, Aspirin, 4-Hydroxybenzoic acid, Sorbic acid, Benzoic acid |
HPLC Analysis of Drug Composition on Heritage C18 Column According to US Pharmacopeia
Conditions of Experiment
Column: | Heritage С18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6 x 100 mm, 5 um, 100A |
Mobile Phase: | MeOH/H2O/AcOH 30/67/3 |
Detection: | UV 275 nm |
Sample: | 0.2 mg/ml |
Injection: | 5 uL |
Flow rate: | 1 mL/min |
Analytes
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetaminophen, Caffeine, Aspirin, Benzoic acid, Salicylic acid |