Bentazon
HPLC Analysis of Herbicide Bentazon on Coresep 100 Mixed-Mode Column
Application description
Bentazon is a thiadiazine herbicide. It is hydrophobic and neutral in nature. Bentazon was retained and analyzed on core-shell mixed-mode Coresep 100 column. The compound is retained by the reversed-phase mechanism. The retention time can be adjusted by changing the amount of the acetonitrile in the mobile phase. This method can be used for analysis of neutral and ionizable herbicides and pesticides in various matrices like soil, wastewaters, crops, etc. The method is compatible with mass spectrometry and is robust with good peak shape and retention control.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 3.0 x 100 mm |
Mobile Phase: | ACN gradient from 15% to 60%, AmFm pH 3 from 30 mM to 80 mM, in 7 min |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.7 mL/min |
Analytes
Class of compounds: | Aromatic compound, Herbicide |
Nature of compounds: | Hydrophobic, Neutral |
Compounds: | Bentazon |