Benzenesulfonic acid Benzenesulfonic acid Benzenesulfonic acid Select Applications HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient HPLC Analysis of 6 Aromatic Alcohols, Acids and Neutral Compounds on Amaze Aromatic Stationary Phases. HPLC Separation of Hydrophobic, Hydrophilic, Basic and Acidic Compounds on Amaze TR Mixed-Mode Column HPLC UV Analysis of Benzenesulfonic, p-Toluenesulfonic and 2-Naphtanesulfonic Acids on Heritage MA Mixed-Mode Column HPLC Analysis of Benzenesulfonic, p-Toluenesulfonic and 2-Naphtanesulfonic Acids with LC/MS Compatible Conditions on Heritage MA Mixed-Mode Column HPLC Analysis of Benzenesulfonic and p-Toluenesulfonic Acids on Amaze TR Mixed-Mode Column HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: reveresed-phase , anion-exchange Column Dimenstions: 4.6x100, 3 um, 100A Mobile Phase: "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " Detection: 205 nm Sample: 0.2-0.5 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid HPLC Analysis of 6 Aromatic Alcohols, Acids and Neutral Compounds on Amaze Aromatic Stationary Phases. Application description Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C18 columns. Amaze PFP PURCHASE LEARN ABOUT Amaze PFP Conditions of Experiment Column: Amaze PFP Separation Modes: reversed-phase, pi-pi Column Dimenstions: 4.6x150 mm, 5 μm, 100A Mobile Phase: 50% ACN with 20 mM AmFm pH 3 Detection: UV 255 nm Sample: 1 mg/ml Injection: 3 μL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Aromatic compound, Organic acid, Organic compound, Organic solvent Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: Benzyl alcohol, Phenol, Benzoic acid, Toluene, Benzenesulfonic acid HPLC Separation of Hydrophobic, Hydrophilic, Basic and Acidic Compounds on Amaze TR Mixed-Mode Column Amaze TR PURCHASE LEARN ABOUT Amaze TR Conditions of Experiment Column: Amaze TR Separation Modes: RP, cation-exchange, anion-exchange Column Dimenstions: 4.6x150 mm, 5 um, 100A Mobile Phase: 30% ACN with 30 mM AmAc pH 5 Detection: UV 275 nm Sample: 0.2-0.3 mg/ml Injection: 3 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic base, Aromatic compound, Catecholamine, Drug, Neurotransmitter, Organic acid, Organic compound, Preservative, Stimulant Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: Norphenylephrine, Ephedrine, 3,4-Dihydroxybenzoic acid, Benzenesulfonic acid, Propylparaben, Propranolol HPLC UV Analysis of Benzenesulfonic, p-Toluenesulfonic and 2-Naphtanesulfonic Acids on Heritage MA Mixed-Mode Column Heritage MA PURCHASE LEARN ABOUT Heritage MA Conditions of Experiment Column: Heritage MA Separation Modes: RP, anion-exchange Column Dimenstions: 4.6x50 mm, 3 um, 100A Mobile Phase: ACN with AmFm pH 3 Detection: UV 255 nm Sample: 0.2 mg/ml Injection: 3 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Organic compound Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: Benzenesulfonic acid, p-Toluenesulfonic acid, 2-Naphthalenesulfonic acid HPLC Analysis of Benzenesulfonic, p-Toluenesulfonic and 2-Naphtanesulfonic Acids with LC/MS Compatible Conditions on Heritage MA Mixed-Mode Column Heritage MA PURCHASE LEARN ABOUT Heritage MA Conditions of Experiment Column: Heritage MA Separation Modes: RP, anion-exchange Column Dimenstions: 4.6x50 mm, 3 um, 100A Mobile Phase: ACN with AmFm pH 3 Detection: UV 255 nm Sample: 0.2 mg/ml Injection: 3 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Organic compound Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: Benzenesulfonic acid, p-Toluenesulfonic acid, 2-Naphthalenesulfonic acid HPLC Analysis of Benzenesulfonic and p-Toluenesulfonic Acids on Amaze TR Mixed-Mode Column Application description Benzenesulfonic and toluenesulfonic acids are common organic acids used as counter-ions in pharmaceutical industry. Both acids are hydrophilic in nature with strong acidic properties. When traditional RP columns are used, poor retention and peak shape are observed due to non-specific secondary interactions. We developed a robust mixed-mode approach for the separation of benzenesulfonic acid and p-toluenesulfonic acid on the Amaze TR reversed-phase anion- and cation-exchange column. Both acids are retained by a weak reversed-phase mechanism and strong anion-exchange mechanism. The elution of these compounds is controlled by the amount of pH of the buffer, concentration of ions and the amount of acetonitrile in the mobile phase. The method is reproducible and effective with a good retention control and peak shape. It is compatible with major detection techniques including mass spectrometry (MS), Evaporative Light Scattering Detection(ELSD), ultraviolet detection and refractive index detection. Amaze TR PURCHASE LEARN ABOUT Amaze TR Conditions of Experiment Column: Amaze TR Separation Modes: reversed-phase, anion-exchange Column Dimenstions: 4.6x50 mm, 5 um, 100A Mobile Phase: 20% ACN with 15 mM AmFm pH 3 Detection: UV 255 nm Sample: 0.3 mg/ml Injection: 3 ul Flow rate: 1 ml/min Analytes Class of compounds: Organic acid, Organic compound Nature of compounds: Acidic, Hydrophilic, Neutral, Polar Compounds: Benzenesulfonic acid, p-Toluenesulfonic acid
